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KMID : 0881720200350030234
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Journal of Food Hygiene and Safety
2020 Volume.35 No. 3 p.234 ~ p.242
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Monitoring of Raw Materials for Commercial Home Meal Replacement Products Using DNA Barcode Information
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Yu Yeon-Cheol
Hong Ye-Won
Kim Jung-Ju
Lee Dong-Ho
Kim Hyung-Soo
Moon Gui-Im
Park Eun-Mi
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Abstract
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In this study, we monitored the raw materials in home-meal replacement (HMR) products, which have shown more than 63% growth in market size for two years. A total of 89 HMR products were purchased and the DNA barcodes of 112 raw materials in the product samples were analyzed. In order to identify the raw material species, a primer set specific for the 16S ribosomal RNA region of each raw material species was amplified. The amplicon was purified and sequenced, and then used to perform a BLAST search provided by the National Institutes of Health (NIH). The species of the raw material was determined by comparing the nucleotide sequences of the species registered in GenBank with identity and match score. Twenty-four species and three genera were identified from 112 raw materials. Three genera were identified at the genus level because a large number of species belonging to the same genus exist within 98% of the identity criteria. The results of the determination were compared with the available raw materials suggested in the Korea Food Code to determine the Korean name and availability of the foods. Six non-listed species were determined to be edible according to information provided by influential domestic and foreign organizations.
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KEYWORD
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Home meal replacement, DNA barcode, Species identification
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